#  NANOCOURSE - Cancer Metabolism: The Metabolomics Perspective 

 



####  calendar\_today Date and Time 

 **October 19, 2022** 

 09:00AM - 03:00PM EDT 

####  pin\_drop Location 

 **Countway L02-025**  



 

 



 

 **THIS IS A THREE SESSION NANOCOURSE, OPEN ONLY TO PARTICIPANTS WHO REGISTER! FOR MORE INFORMATION ABOUT THE COURSE AND HOW TO REGISTER VISIT [HERE](https://curriculumfellows.hms.harvard.edu/classes/cancer-metabolism-metabolomics-perspective-part-two).**

 **SESSION TWO**

 Day two will focus on LC-MS data analysis and statistics. Targeted and untargeted metabolomics platforms will be compared. Participants will work on analyzing metabolomics data using R-code and MetaboAnalyst.

 Participants will:

- Be introduced to theory and practice of data analysis, chromatography peaks alignment, ion adducts, and isotopes.
- Be introduced to TraceFinder (TF) and Compound Discoverer (CD) and targeted vs untargeted LC-MS analysis
- Practice data analysis using TF and CD.
- Demo and practice data integration and statistics using R-code and MetaboAnalyst
- Demo and practice preparation of data for publication using Prism and Illustrator
- Participants will be given homework assignment: Data analysis, interpretation, visualization – communicate your results by preparing a short presentation. Group can present together.

 **Tentative Schedule**

Sort    Time

 

  Task

 

  Notes

 

    9-10pm

 

  LC-MS data analysis intro

 

  Discussion of targeted vs untargeted LC-MS analysis

 Introduction into peak quality and alignment

 

    10-12pm

 

  Data analysis set up

 

  TF and CD will be run remotely. TF for targeted CD for untargeted

 

    12-13:30 pm

 

  Lunch

 

  CD analysis will continue unsupervised over lunch

 

    13:30-3pm

 

  Finalize data analysis. Export data, analyze with R-code, Metaboanalyst and Prism

 

  These steps will be on personal computers

 

 



 Participants can communicate via email to finalize analysis and presentations.



 

 



 

 

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