#  NANOCOURSE - Cancer Metabolism: The Metabolomics Perspective  

 



####  calendar\_today Date and Time 

 **October 17, 2022** 

 09:00AM - 03:00PM EDT 

####  pin\_drop Location 

 **BCH Enders 10**  



 

 



 

 **THIS IS A THREE SESSION NANOCOURSE, OPEN ONLY TO PARTICIPANTS WHO REGISTER! FOR MORE INFORMATION ABOUT THE COURSE AND HOW TO REGISTER VISIT [HERE](https://curriculumfellows.hms.harvard.edu/classes/cancer-metabolism-metabolomics-perspective-part-two).**

   
**SESSION ONE**

 Day one of the course will focus on sample preparation and LC-MS analysis.

 Participants will:

- Be introduced to the necessary concepts and instructed on the steps of sample preparation and analysis by LC-MS. Theory and specifics of chromatography, mass spectrometry and data collection will be discussed.
- Extract diverse samples (cells, livers, brain tissue, plasma) and process them for LC-MS using different conditions
- Write LC-MS methods, prepare chromatography and samples, and run the LC-MS machines.

 **Tentative Schedule**

Sort    Time

 

  Task

 

  Notes

 

    9-10pm

 

  LC-MS and sample prep introduction

 

  Lecture will take place in BCH conference room (TBD)

 

    10-12pm

 

  Sample prep, part 1

 Two groups

 

  Cells, livers, brain tissue, plasma +/- Ellman’s reagent and two-three extraction buffers will be prepared by different student groups

 

    12-13:30 pm

 

  Lunch

 

  Sample prep will continue without attendance

 

    13:30-3pm

 

  Sample prep, part 2

 Write methods, run LC-MS

 

  LC column will be set up, participants will write method and prepare sample pools and LC-MS vials. Participants will start runs

 

 



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