BEGIN:VCALENDAR VERSION:2.0 X-WR-CALNAME;VALUE=TEXT:NANOCOURSE - Cancer Metabolism: The Metabolomics Perspective PRODID:-//Harvard events data//EN BEGIN:VEVENT UID:event_1652910_0 SUMMARY:NANOCOURSE - Cancer Metabolism: The Metabolomics Perspective DESCRIPTION:

THIS IS A THREE SESSION NANOCOURSE, OPEN ONLY TO PARTICIPANTS WHO REGISTER! FOR MORE INFORMATION ABOUT THE COURSE AND HOW TO REGISTER VISIT HERE.

SESSION ONE

Day one of the course will focus on sample preparation and LC-MS analysis.

Participants will:

Be introduced to the necessary concepts and instructed on the steps of sample preparation and analysis by LC-MS. Theory and specifics of chromatography, mass spectrometry and data collection will be discussed.
Extract diverse samples (cells, livers, brain tissue, plasma) and process them for LC-MS using different conditions
Write LC-MS methods, prepare chromatography and samples, and run the LC-MS machines.

Tentative Schedule

Time	Task	Notes
9-10pm	LC-MS and sample prep introduction	Lecture will take place in BCH conference room (TBD)
10-12pm	Sample prep, part 1 Two groups	Cells, livers, brain tissue, plasma +/- Ellman’s reagent and two-three extraction buffers will be prepared by different student groups
12-13:30 pm	Lunch	Sample prep will continue without attendance
13:30-3pm	Sample prep, part 2 Write methods, run LC-MS	LC column will be set up, participants will write method and prepare sample pools and LC-MS vials. Participants will start runs

LOCATION:BCH Enders 10 STATUS:CONFIRMED DTSTART:20221017T130000Z DTEND:20221017T190000Z END:VEVENT END:VCALENDAR