NANOCOURSE - Cancer Metabolism: The Metabolomics Perspective
Date and Time
Location
THIS IS A THREE SESSION NANOCOURSE, OPEN ONLY TO PARTICIPANTS WHO REGISTER! FOR MORE INFORMATION ABOUT THE COURSE AND HOW TO REGISTER VISIT HERE.
SESSION TWO
Day two will focus on LC-MS data analysis and statistics. Targeted and untargeted metabolomics platforms will be compared. Participants will work on analyzing metabolomics data using R-code and MetaboAnalyst.
Participants will:
- Be introduced to theory and practice of data analysis, chromatography peaks alignment, ion adducts, and isotopes.
- Be introduced to TraceFinder (TF) and Compound Discoverer (CD) and targeted vs untargeted LC-MS analysis
- Practice data analysis using TF and CD.
- Demo and practice data integration and statistics using R-code and MetaboAnalyst
- Demo and practice preparation of data for publication using Prism and Illustrator
- Participants will be given homework assignment: Data analysis, interpretation, visualization – communicate your results by preparing a short presentation. Group can present together.
Tentative Schedule
Time | Task | Notes |
9-10pm | LC-MS data analysis intro | Discussion of targeted vs untargeted LC-MS analysis Introduction into peak quality and alignment |
10-12pm | Data analysis set up | TF and CD will be run remotely. TF for targeted CD for untargeted |
12-13:30 pm | Lunch | CD analysis will continue unsupervised over lunch |
13:30-3pm | Finalize data analysis. Export data, analyze with R-code, Metaboanalyst and Prism | These steps will be on personal computers |
Participants can communicate via email to finalize analysis and presentations.