NANOCOURSE - Cancer Metabolism: The Metabolomics Perspective
Date and Time
Location
THIS IS A THREE SESSION NANOCOURSE, OPEN ONLY TO PARTICIPANTS WHO REGISTER! FOR MORE INFORMATION ABOUT THE COURSE AND HOW TO REGISTER VISIT HERE.
SESSION ONE
Day one of the course will focus on sample preparation and LC-MS analysis.
Participants will:
- Be introduced to the necessary concepts and instructed on the steps of sample preparation and analysis by LC-MS. Theory and specifics of chromatography, mass spectrometry and data collection will be discussed.
- Extract diverse samples (cells, livers, brain tissue, plasma) and process them for LC-MS using different conditions
- Write LC-MS methods, prepare chromatography and samples, and run the LC-MS machines.
Tentative Schedule
Time | Task | Notes |
9-10pm | LC-MS and sample prep introduction | Lecture will take place in BCH conference room (TBD) |
10-12pm | Sample prep, part 1 Two groups | Cells, livers, brain tissue, plasma +/- Ellman’s reagent and two-three extraction buffers will be prepared by different student groups |
12-13:30 pm | Lunch | Sample prep will continue without attendance |
13:30-3pm | Sample prep, part 2 Write methods, run LC-MS | LC column will be set up, participants will write method and prepare sample pools and LC-MS vials. Participants will start runs |